DeltaVision OMX Imaging System
DeltaVision OMX is an advanced multimode, super-resolution systems representing the next generation of optical microscopy. DeltaVision OMX systems offer super-resolution modes using 2D and 3D structured illumination (SIM) and localization microscopy, as well as fast widefield acquisition. The innovative Blaze SIM Module offers dynamic high speed SIM, which has made live cell super-resolution imaging a reality. In addition, DeltaVision OMX systems support a novel Ring-TIRF system that offers large, evenly illuminated, fields of view in TIRF mode enabling applications such as single molecule tracking and localization super-resolution imaging.
Objectives: 60x SIM, 60x SIM TIRF, 100x SIM
Cameras: Front Illuminated sCMOS, 2560 x 2160 pixels
Software: OMX Acquistion software version 3.70
Location: BioCity, 5th floor, room 5149
Contact: Jouko Sandholm or Markku Saari
Fluorescence lifetime imaging microscopy, FLIM, is a technique for producing an image based on the differences in the exponential decay rate (lifetime) of the fluorescent molecule.
FLIM is an easy and fast application to image FRET as the lifetime of the donor decreases upon FRET. CFP-YFP and GFP-DsRed pairs have been successfully used.
Our Lambert Instruments LIFA is a fast frequency domain FLIM system, which is attached to an inverted Carl Zeiss AxioImager microscope body. With this system we can image only fixed samples and only in widefield mode. The light source is multi-LED with excitation lines 406, 469 and 533nm. With the LIFA system we can image lifetimes with a resolution < 100 ps in a range of 0.1 ns – 1 ms.
For basic information, please read ‘Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell’ by Bastiaens and Squire. (Trends Cell Biol. 1999 Feb;9(2):48-52.)
In case of needs for FLIM in confocal resolution or with a live cell sample, we can apply an access to Leica SP5 STED imaging platform to perform time domain FLIM (contact: Laboratory of Biophysics, BioCity, 5th floor, room 5086 / Sami Koho).
Total Internal Reflection microscopy (TIRFM) is a technique, which allows imaging of fluorophores near of the basal cell membrane with high resolution in Z-axis. Approximately 70-150 nm axial resolution can be achieved, while in common confocal microscopy the limit is approximately 600nm.
TIRFM is designed for cell membrane studies, such as endosytosis and integrin trafficking. However, it is very suitable for all kind of sensitive wide field microscopy, due to high quality objectives and sensitive cameras.
Our TIRF has two different cameras dedicated to different applications: a very sensitive emccd-camera with a 512×512 chip, and a sensitive and fast high resolution sCMOS camera. The microscope is equipped with incubation elements, motorized stage and Zeiss Definite Focus drift correction device.
The Zeiss SteREO Lumar.V12 fluorescence stereo microscope is an instrument for low-magnification microscopy. Our 0.8x and 1.2x long working distance objectives allow a researcher to perform (for example) dissection using objective lenses permitting a maximal magnification of 120x. The versatility of the instrument permits imaging of entire mouse brain slices or single fluorescent cells, using the same objective.An Eppendorf InjectMan NI2 semi-automatic microinjection device has been installed beside the stereo microscope allowing, for example, injection into zebrafish embryos. Any material of low granularity and size can be microinjected. The most frequently injected materials are DNA, proteins, tracers, and chemicals with or without attached dyes.A manual Eppendorf CellTram Vario microinjector can be used with the motorized InjectMan NI2 holder. Cell Tram Vario is a good tool, when cell suspension are injected into tissues. Minimum volume that can be set: <20 nL/<2 nL (coarse/fine)Cameras: Zeiss AxioCam ICc3 (colour CCD camera), and Zeiss AvioCam Hrm (b/w CCD camera)
FL filters: UV/Dapi, GFP/Alexa488, CY3/DsRed, CFP, YFP
Objectives: NeoLumar 0,8x, NeoLumar 1,2x
Special: Eppendorf microinjection device
Software: Zeiss Zen lightLocation: BioCity, 5th floor, room 5062
Laser-capture microsdissection (LCM) uses laser beams to extract biological material of interest out of a tissue specimen. The Zeiss PALM microdissection device employs a focused beam to cut the desired material, and a defocused beam to catapult the sample into the cap of a sample tube. Cutting accuracy of less than 1 µm can be achieved.
The source material can be placed on a normal objective glass slide or on a membrane slide, depending on the way of approaching. The system can be used with frozen or paraffin-embedded tissue samples, single cells, and even single chromosomes.
The microscope is equipped with a fluorescence system and has a video camera to assist in target visualization.
Available objectives are the FLUAR 10x/0.50 and LD ACHROPLAN 40x/0.60 for dissection, and the LD ACHROPLAN 20x/0.40 for specimen visualization.
Adhesive caps and Zeiss PALM membrane slides can be purchased from CIC.
Location: BioCity, 5th floor, room 5149 / Markku Saari
The AFM is the model 5500 from Agilent (former Molecular Imaging). It can be mounted onto Zeiss Observer (widefield microscope) or Leica DMI6000 (confocal/STED microscope) for simultaneous optical imaging.
This AFM has maximum scan range 100 µm (x&y-axis) and 9 µm (z-axis). It can provide all standard imaging modes (contact, tapping, force modulation conductivity etc.) and soft magnetic AC mode for cells and simultaneous topography / recognition for functional topography mapping.
The probe to be used should be chosen according the needs of your sample.
The system can provide environment control (heating, CO2)
Location: Biocity 5th floor, Department of Anatomy/laboratory of biophysics
Contact person: Näreoja Tuomas, tuonar (at) utu.fi, phone: 02-3337058
JPK Nanowizard II combined with a Carl Zeiss confocal microscope (Zeiss LSM510).
Simultaneous imaging with the optical microscope. Scan range 100 x 100 x 15 μm. BioCell for biological samples under physiological conditions. All standard imaging modes, and a CellHesion module that extends the Z range to 100 μm and enables cell adhesion measurements with a cell attached to the cantilever.
Location: BioCity, floor -1, staircase A / Pasi Kankaanpää.
A compact spinning disc confocal microscope, designed for rapid analyses of fixed and live cell applications in microtiter plates. The included PE Harmony High Content Imaging and Analysis Software (v3.5) allows quantitative evaluation of morphometric image data and automated image analyses.
Operetta has a live cell chamber that maintains environmental conditions (temperature, CO2), and provides a spectrum of kinetic capabilities for time course experiments and observation of cellular dynamics. Allows excitation in the range of 360 – 640 nm. Objectives available: 2x WD, 10x WD, 20x WD, 60x NA
Location: Pharmacity, HCS Laboratory, floor 4, staircase B (Room 4054)
Contact person: Malin Åkerfelt