X-ray crystallography equipment
The following equipment is available for X-ray crystallography:
- Rigaku Micromax 007 HF X-ray generator
- MAR345 Imaging plate detector
- Oxford Cryosystem to allow data collection at cryogenic temperatures (100K)
- Varimax ArcSec optics
- Crystallisation room
- Incubators at 4°, 16° and 23° C for temperature-controlled crystallisations
- Linux PC workstation for data collection and assessment of the diffraction
- Linux workstations for data processing, structure determination, molecular graphics, modeling, structure validation
The Facility has various workstations running under Linux or Unix operating systems. Programs for all the steps of a crystal structure determination (data collection, processing, phasing, refinement, molecular graphics, structure analysis and validation) and modelling are installed and run on each computer. In addition, software for protein structure prediction, protein-ligand and protein-protein docking are locally installed.
Because protein crystallography requires highly purified proteins, we have experience in protein purification strategies that can deliver one-band protein sample in SDS PAGE or a single peak in mass spectrometry. FPLC, HPLC and various chromatographic media are available. A Nanodrop 2000C spectrophotometer is used for protein concentration measurements (in cuvettes and drops).
A variety of crystallisation screens and conditions are available in our Unit to facilitate the crystallisation of new proteins and their complexes. Crystallisation conditions obtained after initial screening are further refined to obtain well-diffracting crystalso suitable for structural studies. Incubators at different temperatures (4o, 16o, 23o C) are available.