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New course starts (2018)

Basic X-ray crystallography techniques: How to solve and understand protein structures (BIOI4303)

Level: Advanced studies

Aim: The aim of the course is to familiarize students and experienced researchers with the various crystallographic programs used in the determination of protein structures. By the end of the course, it is expected that the participants will be able to solve a protein crystal structure from well-diffracting crystals using X-ray crystallography techniques, understand the terminology used in publications and learn the basics of complementary structural biology techniques.

Content: The course consists of lectures, demonstrations and practicals of all major steps in a crystal structure determination – from protein expression, crystallization and data collection to computational techniques and validation of the final structure. Key crystallographic programs include MOSFLM for data processing, CCP4i for data handling and refinement, PHASER for molecular replacement, PHENIX for experimental phasing and refinement, COOT for visualization of the structures and manual rebuilding, and Buccaneer for automated building of protein structures. In addition, the course will cover various bioinformatics and computational tools for structure analysis as well as the use of modern complementary techniques in structural biology (such as neutron scattering, NMR, cryo-EM, SAXS, modelling, ab initio structure predictions) for a better understanding of protein structures and complexes.

Starting date: January 30th, 2018 at 09:00 (K127 computer class, Agora building, University Hill)

Duration: 28 hrs (5 hrs lectures + 23 hrs practicals/demonstrations)

Dates: Tue 30 Jan 9-12 (first lecture-compulsory); Thu 1 Feb (2 hrs demo-time to be arranged); Tue 6 Feb 9-11; Tue 13 Feb 9-12 (setup of computer accounts-presence required); Tue 20 Feb 9-12; Tue 27 Feb 9-12; Tue 6 Mar 9-12; Tue 13 Mar 9-12; Tue 20 Mar 9-12; Tue 27 Mar 9-12

Place: K127 computer class, Agora building, University Hill  (except demo)

Demo: Turku Centre for Biotechnology, X-ray core facility

Evaluation: Attendance (>80%) and a written exam (multiple-choice questions + essay); 4 ECTS; grade 1-5

Responsible person: Tassos Papageorgiou. To enroll for the course please send an e-mail (tassos.papageorgiou (at) btk.fi) by January 27 at the latest with the subject line: X-ray crystallography course or register directly through moodle

Fee: MSc students free; PhD students 100€; experienced researchers 200€

Other information: Maths is kept at minimum level