Information asked for new projects
These are some of the questions we ask before taking up a new project:
- Description of the protein (name, size, monomeric/oligomeric, post-translational modifications, sequence/structural homology to other proteins of known structure)
- Biological relevance and implications (e.g why you want to solve the structure?)
- Preliminary work and current status
-Is cDNA available?
-Have expression vectors been made?
-Has the protein been expressed?
-Is the expressed protein soluble/insoluble?
-Has purification been established?
-Is the expressed protein biologically/enzymatically active?
-How much protein has been purified/produced to date?
-Preferable expression system (host, strains, purification tags)