Information asked for new projects

These are some of the questions we ask before taking up a new project:

  • Description of the protein (name, size, monomeric/oligomeric, post-translational    modifications, sequence/structural homology to other proteins of known structure)
  • Biological relevance and implications (e.g why you want to solve the structure?)
  • Preliminary work and current status

-Is cDNA available?

-Have expression vectors been made?

-Has the protein been expressed?

-Is the expressed protein soluble/insoluble?

-Has purification been established?

-Is the expressed protein biologically/enzymatically active?

-How much protein has been purified/produced to date?

-Preferable expression system (host, strains, purification tags)