Introduction to the laboratories
Food Chemistry and Food Development
The expertise in lipidomics of the Food Chemistry and Food Development unit of the Department of Biochemistry, University of Turku includes qualitative and quantitative analysis of fats, oils and other lipid-soluble bio-active components in foods and biological materials. Our target is in resolving the composition of natural lipids, their oxidized products along with functional genomics of lipids. Our lipidomics expertise ranges from fatty acid composition analysis (GC-FID) and equivalent carbon number distribution (LC-LSD), to fatty acid combinations, triacylglycerol fatty acid regioisomerism (LC/APCI-MS, LC/ESI-MS/MS and direct inlet ammonia NICI-MS/MS) and stereoisomerism analysis (chiral chromatography combined with LSD or MS/MS) as well as analysis of oxidized lipid species
Metabolomics is an important tool in molecular food research including food analysis, food development research, and nutritional intervention studies. We analyse metabolic phenotypes from various food matrices and human biofluids using both untargeted and targeted metabolomics using 1H NMR. We especially aim to understand the effects of Northern growth latitudes and innovative processing technologies on the composition and health impacts of foods. Also, we apply NMR metabolomics to study the human metabolomic status from plasma, serum, urine and feces samples after clinical dietary interventions.
Our metabolomics research also includes the targeted analyses of polar metabolites in foods derived from plants. Our aim is to create deep understanding of different groups of compounds that contribute to the sensory properties and biological activities of foods, for example example, sugars, acids, phenolics, and aroma compounds. The methods commonly used are chromatography coupled with mass spectrometry (UHPLC-DAD, UHPLC-MS/MS, GC-FID, GC-MS/MS)) and targeted and non-targeted NMR.
The facilities located in the Food Chemistry and Food Development unit include GC-FID, GC-O-FID, GC-MS, GC-MS/MS, GC-MS/MS, HPLC-DAD, UPLC-DAD, UPLC-MS/MS. Other detectors that can be couple to the H(U)PLC are light scattering detector and florescence detector. We also have a sensory analysis lab for research in flavor chemistry and food development.
For more information, please visit our website: http://www.utu.fi/fi/yksikot/sci/yksikot/biokemia/tutkimus/ekeh/Sivut/home.aspx
Turku PET Centre
Our research is focused on imaging tumor energy metabolism, hypoxia, amino acid transport and inflammation. The main objectives are to a) develop and validate PET tracers for imaging of the tumor microenvironment, b) achieve a deeper understanding of biomolecular mechanisms that regulates the uptake of PET tracers used in oncological imaging and c) image microenvironmental changes in tumors during RT treatment.
Our ongoing projects are:
Imaging of hypoxia during radiotherapy treatment
Imaging of inflammation in cancer
Link between hypoxia, TLR9 and HPV in head and neck cancer
Effect of hypoxia on the uptake of amino acid based PET tracers
For more information, please visit our website: http://turkupetcentre.fi/
NMR Instrument Centre