“Use of the Viral Vectors Core” at CBT requires that your research supervisor has obtained permission from the GTLK. The permission including risk assessment must be given to the staff at the Viral Vector Core before access will be given. It is not permitted to work with viruses at CBT outside of this approved work space.
Virus Lab Safety Rules
Download a pdf of Virus Lab Safety Rules
The virus lab is a restricted access area for work with replication-incompetent viruses only. Biosafety Level 2 conditions should be used at all times when handling viruses and transduced cells.
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I. PERSONAL SAFETY
- Only approved persons (i.e. working on projects approved by Valvira) can enter and use the virus lab. The supervisor is responsible for safety issues regarding viral vector use by their lab members.
- Every worker intending to work in the virus lab must obtain electronic access. This form must be signed by the group leader responsible for the virus core.
- Before permission is given, the new worker visits the virus lab with Ketlin Adel and is guided on correct work procedures in the lab.
- A blood sample is taken from new workers before they commence with virus work (0 sample).
- A personal lab coat and overshoes should be worn on entry to the virus lab.
- All viral work must be done in the laminar flow cabinet.
- Large-scale spills and accidents should be reported to the viral core staff.
II. BASIC RULES FOR WORK WITH VIRUSES
- It is not permitted to work with viruses in the BSL1 labs in CBT. Every group using the BSL2 virus lab must first obtain permission from the Gene Technology board for all vectors used. A copy of this permission must be given to the virus core staff before commencing work in the lab. Project information is confidential.
- The virus lab is designated for work with replication-deficient lentiviruses (LV) (2nd and 3rd generation vectors) and adenoviruses (Ad). Work with replication-competent viruses is not permitted. Work with other viruses or with vectors bearing oncogenes should be reported to the virus core staff in advance.
- Every use of the virus lab must be entered in the log book.
- To avoid cross-contamination, laminar flow cabinets and incubators are designated for ‘LENTIVIRUS’ or ‘ADENOVIRUS’.
- Virus-containing material (e.g. virus preps, transduced cells) must not be removed from the BSL2 lab without inactivation unless for ultracentrifugation, in which case ask the technical staff to show you the correct procedure. Material in tissue culture dishes must be inactivated appropriately (see below) before disposal to GMOII waste.
III. DECONTAMINATION BEFORE DISPOSAL
- Clean work surfaces after completing work using 1-2% Virkon (note solution must be coloured to be active). Note!! Inactivate lentiviruses using 70% ethanol. Inactivate adenoviruses using 1-2% virkon. (Adenovirus is not destroyed with ethanol).
- To inactivate contaminated media, pour it into the container with concentrated bleach (sodium hypochlorite). All contaminated media will be autoclaved before disposal by the virus core personnel.
- Used plastic ware should be closed before disposal. Tips, serological pipettes, filters, syringes should be in a sealed container before disposal in GMOII waste. e.g. place used tips in culture flasks and seal other items in plastic bags provided in the virus lab.
- Liquid waste is collected in bottles containing hypochlorite. This waste is autoclaved by virus core personnel before disposal.
Virus core responsible person:
Eleanor Coffey, email@example.com, (02)333 8605